BPC-157 + TB-500 (Wolverine Blend)

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The Wolverine Blend is a research-use-only combination of two synthetic peptides—BPC-157, a 15-amino-acid pentadecapeptide, and TB-500, a 7-amino-acid acetylated fragment—supplied together as a single lyophilized preparation for in-vitro laboratory investigation.

What is BPC-157 + TB-500 (Wolverine Blend)?

The "Wolverine Blend" is a co-formulated research preparation pairing two distinct synthetic peptides that are frequently studied side by side in tissue-repair and regenerative research models. The first component, BPC-157, is a pentadecapeptide of 15 amino acids with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. It carries the molecular formula C62H98N16O22, a molecular weight of 1419.55 g/mol, and CAS number 137525-51-0. BPC-157 is derived from a partial sequence related to a gastric protein and is characterized by its proline-rich central region.

The second component, TB-500, is a shorter synthetic fragment of 7 amino acids with the acetylated sequence Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH. It has the molecular formula C38H68N10O14, a molecular weight of 889.02 g/mol, and CAS number 885340-08-9. TB-500 corresponds to an actin-binding region associated with the thymosin beta-4 family of peptides and is studied in the context of cell migration and cytoskeletal dynamics in laboratory systems.

Classified within the blends category of research chemicals, this material is offered strictly for in-vitro and laboratory research applications. Both peptides are commonly referenced together in academic literature examining extracellular matrix organization, angiogenesis models, and cellular motility. The compound is intended solely as an analytical reference and experimental input; it is not a drug, supplement, or article for human or veterinary use, and no physiological outcomes are claimed or implied.

Reconstitution & handling

As supplied, the Wolverine Blend is a lyophilized (freeze-dried) powder containing both peptides and is reconstituted immediately before laboratory use. Bacteriostatic or sterile water is the conventional solvent for handling peptide powders of this type; the diluent is typically introduced slowly against the inner wall of the vial and allowed to dissolve without vigorous agitation. Both BPC-157 and TB-500 are water-soluble peptides, so gentle swirling rather than shaking is used to bring the material fully into solution while minimizing mechanical shear and foaming that can stress peptide bonds.

Because the preparation combines two peptides of differing size and composition, researchers generally allow adequate time for complete dissolution and inspect the solution for clarity before proceeding. Reconstitution is best performed under clean conditions to preserve integrity, and the resulting solution should be treated as a working stock rather than a finished formulation. All handling described here pertains to laboratory chemistry only and involves no administration guidance of any kind.

Storage & stability

In its lyophilized state, the blend is stable when stored cold, and long-term storage is typically at or below -20°C, protected from light and moisture, where the dry powder retains integrity for extended periods. Once reconstituted, peptide solutions are considerably less stable and are held refrigerated at approximately 2–8°C for short-term laboratory use, with minimal exposure to repeated freeze-thaw cycles, elevated temperature, and light. Aliquoting a reconstituted stock into single-use portions is a common practice to preserve quality and reduce degradation across the study period.

How it's tested

Identity and purity of the Wolverine Blend are verified analytically before release. Reversed-phase high-performance liquid chromatography (HPLC) is used to confirm a purity of ≥99% and to characterize the peptide profile of each component. Mass spectrometry (MS) is applied to confirm molecular identity against the expected molecular weights—1419.55 g/mol for BPC-157 and 889.02 g/mol for TB-500—ensuring the correct sequences are present. A Certificate of Analysis (COA) documenting these results is available for the material, providing traceable confirmation of identity and purity for research recordkeeping.

Frequently asked questions

What exactly is the Wolverine Blend?

It is a research-use-only preparation combining two synthetic peptides—BPC-157 (a 15-amino-acid pentadecapeptide) and TB-500 (a 7-amino-acid acetylated fragment)—supplied together as a single lyophilized powder for in-vitro laboratory study.

What are the molecular details of each component?

BPC-157 has the formula C62H98N16O22, molecular weight 1419.55 g/mol, CAS 137525-51-0, and sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. TB-500 has the formula C38H68N10O14, molecular weight 889.02 g/mol, CAS 885340-08-9, and sequence Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH.

How is the blend reconstituted?

The lyophilized powder is dissolved with a suitable sterile or bacteriostatic aqueous solvent added slowly down the vial wall. Both peptides are water-soluble, so the vial is swirled gently rather than shaken until the solution is clear.

How should the material be stored?

Store the lyophilized powder cold, typically at or below -20°C, away from light and moisture. Reconstituted solution is kept refrigerated at about 2–8°C, aliquoted where practical, and protected from repeated freeze-thaw cycles.

How is purity and identity verified?

Purity of ≥99% is confirmed by reversed-phase HPLC, and molecular identity is confirmed by mass spectrometry against the expected weights of each peptide. A Certificate of Analysis (COA) is available.

Is this product intended for human or animal use?

No. The Wolverine Blend is sold strictly for laboratory research use only. It is not a drug, supplement, or article for human or veterinary use, and no physiological effects are claimed.